SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. In the lower half of the window, left side, there is a box labeled reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Wide range of applications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. easy to use and read multiple plates very quickly. 2. were mixed for 2 minutes using a plate shaker. The chamber. Driver installation. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9660-665 Eskdale Road. The M5e Reader was specifically requested by researchers. 18 x 14 x 8 ins. Choose from a three- (M3), four- (M4), orThe SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. HTRF is a. For SpectraMax L or LMax II, LMax II. SpectraMax ABS Plus SpectraTest ABS2 v1. . Shop now. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. In the M2, M5 and Plus 384, the light. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 2. The SpectraMax M5 is a multi-detection, monochromator-based reader with both topand bottom-read capability. Spectramax Softmax Pro 6. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. If no sound is heard during initialization, contact technical support. The chamber. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Reader Documentation Version No. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 2 only) SpectraMax M-series Microplate readers. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Sunnyvale, California -- October 5, 2017. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Temperature Regulation. With optimized reagents, option to operate the readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 1. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. Gemini XPS. 383 inches whereas the maximum allowed is 5. #3635 or Greiner cat. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Example: [Drawer]. 0200-7027. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Your applications, your modes, your choice. Align the two slots on the bottle holder with the two pegs on the rail. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Last lab had ours for years and never had a problem with it. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. used. 8. 2. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. 2. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. PURPOSE. PERKIN ELMER Wallac Victor 2V Microplate Reader. es •. Molecular Devices Spectramax M5 Microplate Reader; Related products. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. edu, 814-863-3503. 2. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. SpectraMax M2, M2e;. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance,. Abs). Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Compatible Fluorescence Polarization Plate Readers. 6% for the on-board optics (monochromators). Supplier: Molecular Devices. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 00 USD. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Type Clear: Add to Cart. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Comes from Lab closure Approx. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Description. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Last lab had ours for years and never had a problem with it. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. 5 pM (0. The assay window spanned four orders of magnitude. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. For more information, please visit us at Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Accordingly there are different configurations possible. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Pathcheck Pathlength Measurement Technology. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Detection modalities. spr SpectraMax iD3 SpectraTest ABS2 v1. Winnersh Triangle. Access this by going to Help >. TR-FRET assay principle. Other technologies such as imaging. 3: The cuvette chamber. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The calculated LOQ values were 0. • Tel. 95. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. M5 E -HTRF Readers. D 49 7. For example, microplates containing different samp les can be read using the same orThe Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Some familiarity with Windows should be sufficient to use it. Multimode Reader Comparison Chart. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Results. Click SoftMax Pro Software User Guide. both cuvette-based and microplate reader applications SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 筛选时,SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机,允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长,而无需额外再选购滤光片配件。这篇应 FlexStation® 3 Multi-Mode Microplate Reader. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. BENEFITS • No filters needed • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. book Page 21 Wednesday, July 7, 2010 2:09 PM. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. $6,649. Manufacturer: Molecular Devices Model: SpectraMax Plus 384 The SpectraMax ® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Following treatment the samples were assayed for protease activity in triplicate using the Pierce Fluorescent Protease Assay Kit (Thermo Scientific, Waltham USA),. You can also interpret your results using integrated data analysis software. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Analyst, M5, Flex 3, and SpectraMax L readers work differently. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. However, when a specific probe is not available, or it causes off-target effects on the. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. 314 low lamp level warning. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper. Compact and tunable absorbance microplate readers DATA SHEET. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Reader Documentation Version No. 筛选时,SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机,允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长,而无需额外再选购滤光片配件。这篇应FlexStation® 3 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 Microplate Reader is available from The Lab World Group. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. It features a modular design that allows users to add or change detection cartridges according to their needs. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax M2 and M2e are versatile and reliable. Instructions. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. *This plate definition in SoftMax Pro Software is the same for black and white plates. NIST-traceable solution for validating the SpectraMax 340PC384 system. . Pathcheck Pathlength Measurement Technology. Click the OK button. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax M5 e reader has the same performance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF. edu, 814-863-3503. 2018. SoftMax Pro. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. 7°C (gradient heating design). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). ". OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. The guide also covers the software features, the specifications, and the accessories of the systems. Catalog Number: M5. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 1321N1, a human astrocytoma cell line, expresses. Multimode Readers Comparison Chart. . View the Molecular Devices website. When the correct COM port is selected, the icon of the SpectraMax L reader in the upper left hand corner will be displayed in color. free tracer decreases, resulting in an. Fluorescein 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. com. The plate reader can measure multiple readouts, including UV. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. Fill a clean plate with water. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. SpectraMax M5 reader SpectraMax M5e. SpectraMax M5. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. 04 to 3. M3-M5e_manual. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 4. The optical systemsSoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orThe Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). 40266 Email: [email protected] only) SpectraMax M-series Microplate readers. Manufacturer: Molecular Devices. (128) 100%. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Cells in each. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Parts Only. : Please ensure that the plate dimensions entered in the SoftMax Pro software Acquisition Settings (Read Settings > Plate Type > Edit) correspond to those on the specific microplate data sheet, provided by the plate manufacturer. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 9. The plate was vigorously mixed and incubated for 4 min at room temperature in the dark. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. After the plate is read, data will appear in the plate section: 10. Description The Onboard Microprocessor. No additional manuals are available. The SpectraMax ® i3x. Italic and boldface type are used for emphasis. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. 303 unable to cal dark current. 8. The Spectr. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. techsupport. flexibility of monochromator-based systems. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Reader Documentation Version No. Instructions on the proper use of the SpectraMax M5 Plate Reader. Optics. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. com. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. New Life Scientific backs all orders with a 90-day warranty. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. 00. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Choose from a three- (M3), four-. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. begin reading microplates with seven mouse clicks. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 40266 Email: drugdiscoverytech@lifetech. Reader Documentation Version No. 40266 Email: drugdiscoverytech@lifetech. Shake. The following document is intended. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. A solution for low volume applications. It does not work as expected. IMAP assays are based on binding of. Cell-based experiments In the first experiment, we prepared a dilutionThe integration time suggested is 5-10 sec. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. ZERO BIAS - scores, article reviews, protocol conditions and morePage 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Read More. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. After the plate is read, data will appear in the plate section: 11. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. High-performance. The plate was incubated at room temperature for one hour. Figure 10: The Plate section Template Editor, showing the series set up. 1. . 1) Select the correct plate and the wells that you want to measure. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Trends in Immunology. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 000 to 3. SpectraMax® M5 Multi-Mode Microplate Re. Add to Cart. Validation test plates for Abs, FI, Lum optical performance. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. The dual monochromator optics allow the. edu The content of this site is published by the site owner(s) and. This five-mode microplate reader with automatic filter identification. l. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Instrument setup and sample analysis. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. 9000-0756. The SoftMax® Touch Software is installed in. MICROPLATE READER: Used, in good condition, working microplate reader. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Supersedes 1. MichiganSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 00. For more information, please visit us at microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Application Area: Multi mode plate reader. S. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 8. Standard features include a cuvette port, spectral scanning in 1 nm increments, and. For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. While they play an important role in generating data from a wide range of. 0 out of 5 $ 3,799. UV/Vis Photometric Performance: Wavelength Range. For the BioVision cytotoxicity kit, 100 µL of culture medium was removed from each treated well and transferred to a solid white plate. columns 1, 3, 5, 7, 9 and 11 were seeded with 200,000 mock-transfected cells per well. The drawing shows a standard microplate of 15 mm height covered with a lid. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. ". [email protected] M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Category 2 and 3 microplate readers use gradient heating as well. plate analysis, custom calculations, and personalized reporting. 000000 out of 5. Figure 1. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Update Driver. Table 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . 标准功. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Consider these features as well as all of the intended applications when choosing an appropriate luminescence reader for the lab. Description 1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Specifications Plate mixing modes Selectable: off, once prior to any reading, and once prior to and between kinetic readings Plate mixing duration Selectable: 0 to 999 seconds (three-second default. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax M3, M4, M5, and M5 e. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Delivers single mode reader performance in one multimode reader package. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi.